MycorrhizaeV1, Main, Exploration, bibRecord, 003505

Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.

Identifieur interne : 003505 ( Main/Exploration ); précédent : 003504; suivant : 003506

Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.

Auteurs : Roger T. Koide [États-Unis]

Source :

Methods in enzymology [ 0076-6879 ] ; 2005.

RBID : pubmed:15865961

Descripteurs français

KwdFr :
- ADN fongique (isolement et purification), Acides nucléiques (isolement et purification), Champignons (isolement et purification), Microbiologie du sol (MeSH), Mycorhizes (isolement et purification), Méthodes (MeSH), Plantes (microbiologie), Symbiose (MeSH), Écosystème (MeSH).
MESH :
- isolement et purification : ADN fongique, Acides nucléiques, Champignons, Mycorhizes.
- microbiologie : Plantes.
- Microbiologie du sol, Méthodes, Symbiose, Écosystème.

English descriptors

KwdEn :
- DNA, Fungal (isolation & purification), Ecosystem (MeSH), Fungi (isolation & purification), Methods (MeSH), Mycorrhizae (isolation & purification), Nucleic Acids (isolation & purification), Plants (microbiology), Soil Microbiology (MeSH), Symbiosis (MeSH).
MESH :
- chemical , isolation & purification : DNA, Fungal, Nucleic Acids.
- isolation & purification : Fungi, Mycorrhizae.
- microbiology : Plants.
- Ecosystem, Methods, Soil Microbiology, Symbiosis.

Abstract

Mycorrhizal fungi are among the most common symbioses found in terrestrial ecosystems, both natural and managed. They are important for many reasons, but most notably because of their positive effects on plant growth, which are mediated by their uptake of nutrients from the soil and transport of these to the roots. Moreover, many edible fungi are mycorrhizal. The study of mycorrhizal fungi has been hampered by the inability to identify species and individuals in the soil. This has been greatly aided by DNA-based methods, which first require the extraction of DNA. Herein, I discuss some general concerns that must be considered when extracting and purifying DNA from ecological samples and offer specific methods for soil, mycorrhizal roots, and fruiting bodies. These methods are rapid, safe, effective, relatively inexpensive, and convenient because they are based on commercially available kits.

DOI: 10.1016/S0076-6879(05)95005-2
PubMed: 15865961

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 003563
to stream Main, to step Curation: 003563

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.</title>
<author><name sortKey="Koide, Roger T" sort="Koide, Roger T" uniqKey="Koide R" first="Roger T" last="Koide">Roger T. Koide</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802</wicri:regionArea>
<orgName type="university">Université d'État de Pennsylvanie</orgName>
<placeName><settlement type="city">University Park (Pennsylvanie)</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:15865961</idno>
<idno type="pmid">15865961</idno>
<idno type="doi">10.1016/S0076-6879(05)95005-2</idno>
<idno type="wicri:Area/Main/Corpus">003563</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003563</idno>
<idno type="wicri:Area/Main/Curation">003563</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003563</idno>
<idno type="wicri:Area/Main/Exploration">003563</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.</title>
<author><name sortKey="Koide, Roger T" sort="Koide, Roger T" uniqKey="Koide R" first="Roger T" last="Koide">Roger T. Koide</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802</wicri:regionArea>
<orgName type="university">Université d'État de Pennsylvanie</orgName>
<placeName><settlement type="city">University Park (Pennsylvanie)</settlement>
<region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Methods in enzymology</title>
<idno type="ISSN">0076-6879</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Fungal (isolation & purification)</term>
<term>Ecosystem (MeSH)</term>
<term>Fungi (isolation & purification)</term>
<term>Methods (MeSH)</term>
<term>Mycorrhizae (isolation & purification)</term>
<term>Nucleic Acids (isolation & purification)</term>
<term>Plants (microbiology)</term>
<term>Soil Microbiology (MeSH)</term>
<term>Symbiosis (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (isolement et purification)</term>
<term>Acides nucléiques (isolement et purification)</term>
<term>Champignons (isolement et purification)</term>
<term>Microbiologie du sol (MeSH)</term>
<term>Mycorhizes (isolement et purification)</term>
<term>Méthodes (MeSH)</term>
<term>Plantes (microbiologie)</term>
<term>Symbiose (MeSH)</term>
<term>Écosystème (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Fungal</term>
<term>Nucleic Acids</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fungi</term>
<term>Mycorrhizae</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN fongique</term>
<term>Acides nucléiques</term>
<term>Champignons</term>
<term>Mycorhizes</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Plantes</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plants</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Ecosystem</term>
<term>Methods</term>
<term>Soil Microbiology</term>
<term>Symbiosis</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Microbiologie du sol</term>
<term>Méthodes</term>
<term>Symbiose</term>
<term>Écosystème</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Mycorrhizal fungi are among the most common symbioses found in terrestrial ecosystems, both natural and managed. They are important for many reasons, but most notably because of their positive effects on plant growth, which are mediated by their uptake of nutrients from the soil and transport of these to the roots. Moreover, many edible fungi are mycorrhizal. The study of mycorrhizal fungi has been hampered by the inability to identify species and individuals in the soil. This has been greatly aided by DNA-based methods, which first require the extraction of DNA. Herein, I discuss some general concerns that must be considered when extracting and purifying DNA from ecological samples and offer specific methods for soil, mycorrhizal roots, and fruiting bodies. These methods are rapid, safe, effective, relatively inexpensive, and convenient because they are based on commercially available kits.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15865961</PMID>
<DateCompleted><Year>2005</Year>
<Month>08</Month>
<Day>08</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0076-6879</ISSN>
<JournalIssue CitedMedium="Print"><Volume>395</Volume>
<PubDate><Year>2005</Year>
</PubDate>
</JournalIssue>
<Title>Methods in enzymology</Title>
<ISOAbbreviation>Methods Enzymol</ISOAbbreviation>
</Journal>
<ArticleTitle>Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.</ArticleTitle>
<Pagination><MedlinePgn>58-72</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Mycorrhizal fungi are among the most common symbioses found in terrestrial ecosystems, both natural and managed. They are important for many reasons, but most notably because of their positive effects on plant growth, which are mediated by their uptake of nutrients from the soil and transport of these to the roots. Moreover, many edible fungi are mycorrhizal. The study of mycorrhizal fungi has been hampered by the inability to identify species and individuals in the soil. This has been greatly aided by DNA-based methods, which first require the extraction of DNA. Herein, I discuss some general concerns that must be considered when extracting and purifying DNA from ecological samples and offer specific methods for soil, mycorrhizal roots, and fruiting bodies. These methods are rapid, safe, effective, relatively inexpensive, and convenient because they are based on commercially available kits.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Koide</LastName>
<ForeName>Roger T</ForeName>
<Initials>RT</Initials>
<AffiliationInfo><Affiliation>Department of Horticulture, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Methods Enzymol</MedlineTA>
<NlmUniqueID>0212271</NlmUniqueID>
<ISSNLinking>0076-6879</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009696">Nucleic Acids</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017753" MajorTopicYN="N">Ecosystem</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008722" MajorTopicYN="N">Methods</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009696" MajorTopicYN="N">Nucleic Acids</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>5</Month>
<Day>4</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>8</Month>
<Day>9</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>5</Month>
<Day>4</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15865961</ArticleId>
<ArticleId IdType="pii">S0076687905950052</ArticleId>
<ArticleId IdType="doi">10.1016/S0076-6879(05)95005-2</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Pennsylvanie</li>
</region>
<settlement><li>University Park (Pennsylvanie)</li>
</settlement>
<orgName><li>Université d'État de Pennsylvanie</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Koide, Roger T" sort="Koide, Roger T" uniqKey="Koide R" first="Roger T" last="Koide">Roger T. Koide</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003505 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003505 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    MycorrhizaeV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:15865961
   |texte=   Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:15865961" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MycorrhizaeV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 15:34:48 2020. Site generation: Wed Nov 18 15:41:10 2020

	Serveur d'exploration sur la mycorhize
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la mycorhize

Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.

Nucleic acid isolation from ecological samples--fungal associations, mycorrhizae.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki